Transcriptionally active transposable elements in recent hybrid sugarcane

• DOI: 10.1111/j.1365-313x.2005.02579.x
• Publication Date: 2005-11-09T11:08:07Z
• Authors: Paula G. de Araujo; Magdalena Rossi; Erika M. de Jesus; Nilo L. Saccaro; Daniela Kajihara; Rogério Massa; Juliana Maria de ...; Rodrigo Duarte Dr...; Maria C. Falco; Sabrina M. Chabregas; Eugênio C. Ulian; Marcelo Menossi; Marie‐Anne Van Sluys
• Keywords: Callus; Polyploid; clone (Java method)

Summary Transposable elements (TEs) are considered to be important components of the maintenance and diversification genomes. The recent increase in genome sequence data has created an opportunity evaluate impact these active mobile on evolution plant Analysis sugarcane transcriptome identified 267 clones with significant similarity previously described TEs. After full cDNA sequencing, 68 TE were assigned 11 families according their best alignment against a fully characterized element. Expression was further investigated through combined study utilizing electronic Northerns, macroarray, transient stable transformation. Newly synthesized probes from flower, leaf roll, apical meristem callus tissues confirm previous results. Callus as tissue highest number TEs being expressed, revealing that culture drastically induced expression different elements. No tissue‐specific family identified. Different representatives within displayed differential patterns, showing each presented almost every tissue. Transformation experiments demonstrated most Hopscotch clone‐derived U3 regions are, indeed, promoters, although under strong transcriptional regulation. This is large‐scale about pattern indicates genetic transcriptionally highly polyploid complex genome.