Manipulation of plant innate immunity and gibberellin as factor of compatibility in the mutualistic association of barley roots with Piriformospora indica

• DOI: 10.1111/j.1365-313x.2009.03887.x
• Publication Date: 2009-04-07T01:45:54Z
• Authors: Patrick Schäfer; Stefanie Pfiffi; Lars M. Voll; Doreen Zajic; Peter M. Chandler; Frank Waller; Uwe Scholz; Jörn Pons‐Kühnemann; Sophia Sonnewald; Uwe Sonnewald; Karl‐Heinz Kogel
• Keywords: Plant hormone; Strigolactone

Summary Fungi of the order Sebacinales (Basidiomycota) are involved in a wide spectrum mutualistic symbioses with various plants, thereby exhibiting unique potential for biocontrol strategies. Piriformospora indica , model organism this fungal order, is able to increase biomass and grain yield crop induces local systemic resistance diseases tolerance abiotic stress. To elucidate molecular basis root colonization, we characterized interaction P. barley roots by combining global gene expression profiling, metabolic genetic studies. At level, show that colonization reduces availability free sugars amino acids tip. transcriptional consecutive stages covering pre‐penetration‐associated events progressing through showed differential regulation signal perception transduction components, secondary metabolism, genes associated membrane transport. Moreover, observed stage‐specific up‐regulation phytohormone mainly encompassing gibberellin, auxin abscisic acid, but salicylic acid‐associated was suppressed. The changes hormone homoeostasis were accompanied general suppression plant innate immune system. Further studies reduced mutants impaired gibberellin synthesis as well perception, implicate modulator root’s basal defence. Our data further reveal complexity compatibility mechanisms host–microbe interactions, identify signaling target successful fungi.