Free and conjugated polyamines during de novo floral and vegetative bud formation in thin cell layers of tobacco

• DOI: 10.1111/j.1399-3054.1987.tb02842.x
• Publication Date: 2006-05-01T00:28:38Z
• Authors: Patrizia Torrigiani; Maria Maddalena A...; Gabriella Pasqua; Barbara Monacelli; Donatella Serafin...; Nello Bagni
• Keywords: Pedicel; Calyx; Polyamine; Vegetative reproduction; Primordium; Bud

The concentrations of three classes polyamines, trichloroacetic acid‐soluble (free), TCA‐soluble conjugated (to small molecules) and TCA‐insoluble macromolecules), was examined during de novo floral vegetative bud formation in thin cell layers Nicotiana tabacum L. cv. Samsun. Explants (consisting 5–6 epidermal, subepidermal parenchyma cells) were excised either from pedicels or stem internodes at the unripe fruit stage cultured on same medium. In former, first formed flower buds appeared day 8 culture, while latter domes 10. both cases number increased up to 12 15, respectively. Changes dry weight determined throughout culture period. Free putrescine titer 5–60 times types polyamines examined; spermidine content also increased, spermine, when present, did not show significant changes. most abundant, being about 2‐fold ones 10‐fold free ones. major increment occurred internode explants developing buds. pedicel maximum level reached before (emergence with calyx initials), 12, emergence leaf primordia. While prevailed 0, abundant polyamine differentiation processes. rapidly immediately after onset culture. Thus especially putrescine, only ones, seem be involved reproductive phases tobacco growth development.