Role of anti‐Naka antibody, monocytes and platelets in the development of transfusion‐related acute lung injury

• DOI: 10.1111/j.1423-0410.2008.01095.x
• Publication Date: 2008-08-14T03:45:47Z
• Authors: F. Nakajima; M. Nishimura; S. Hashimoto; H. Okazaki; K. Tadokoro
• Keywords: NAK; Monocyte; CD36

Transfusion-related acute lung injury (TRALI) is one of the most serious side-effects transfusion. We report here first two cases TRALI caused by anti-Nak(a) (anti-CD36) antibody from a single blood donor. The aim this study was to clarify role in development.Human microvascular endothelial cells were co-cultured with Nak(a)-positive monocytes and platelets together serum prepared products TRALI-caused antibody-carrying Expressions leukotriene B(4) (LTB(4)) tumour necrosis factor alpha (TNF-alpha) co-culture supernatants determined.The expressions LTB(4) TNF-alpha found be markedly increased, particularly presence both platelets. these mediators almost completed within 4 h after initiation co-culture. Monocyte contribution seemed stronger than that In absence human endothelial, no significant or release observed.Anti-Nak(a) may strongly implicated dysfunctions lead monocyte- platelet-dependent manner.