Structure determination of oligosaccharides isolated from Cad erythrocyte membranes by permethylation analysis and 500‐MHz 1H‐NMR spectroscopy

• DOI: 10.1111/j.1432-1033.1985.tb08628.x
• Publication Date: 2005-03-03T23:35:57Z
• Authors: Françoise HERKT; José Paz PARENTE; Yves LEROY; Bernard FOURNET; Dominique BLANCHARD; Jean‐Pierre CARTRON; Herman van HALBEEK; Johannes F. G. VL...
• Keywords: Oligosaccharide; Borohydride; Glycophorin

Alkaline borohydride reductive cleavage (beta-elimination) of glycophorin A isolated from one individual the rare blood group Cad, resulted in release six acidic oligosaccharide-alditols which were separated by high-performance liquid chromatography (HPLC) on an alkyl amine silicagel column. The structure four them has been determined application methanolysis, methylation analysis and 1H-NMR spectroscopy at 500 MHz. structures relative amounts as follows: oligosaccharide 1: NeuAc(alpha 2-3)Gal(beta 1-3)GalNAc-ol (3.5%); 3: GalNAc(beta 1-4)[NeuAc(alpha 2-3)]Gal(beta (10.5%); 5: 1-3)[NeuAc(alpha 2-6)]GalNAc-ol (10.4%); 6: (71.2%). two other oligosaccharides (2 4) obtained very low amount. major pentasaccharide (oligosaccharide 6) carries Cad determinant is a potent inhibitor human anti-Sda antibody.