Structural Characterization of the N‐Linked Carbohydrate Chains of the Zona Pellucida Glycoproteins from Bovine Ovarian and Fertilized Eggs

• DOI: 10.1111/j.1432-1033.1996.0448h.x
• Publication Date: 2004-07-23T10:29:07Z
• Authors: Toshiyuki Katsumata; Satoru Noguchi; Naoto Yonezawa; Masaru Tanokura; Minoru Nakano
• Keywords: Zona pellucida glycoprotein; Oligosaccharide; Sialidase

The N‐linked oligosaccharides that were released by hydrazinolysis from glycoproteins of zonae pellucidae bovine ovarian eggs, composed neutral (23%) and acidic (77%) carbohydrate chains; almost all the chains neutralized sialidase digestion. Sugar mapping analysis pyridylaminated reverse‐phase normal‐phase HPLC 500‐MHz 1 H‐NMR spectroscopy revealed major chain is a high‐mannose‐type oligosaccharide are di‐, tri‐, tetra‐antennary, fucosylated complex‐type have N ‐acetyllactosamine repeats in non‐reducing regions. structures core regions zona proteins fertilized which obtained vitro fertilization method, essentially same as those egg zonae. amount, however, decreased to 32 mol/100 mol zonae, suggests oocyte during operates on glycoproteins.