A study of two mutant strains of pseudorabies virus (PRV) unable to express thymidine kinase (TK) function

• DOI: 10.1111/j.1439-0450.1995.tb00675.x
• Publication Date: 2010-05-14T03:45:44Z
• Authors: M. Ferrari; G. L. Gualandi; M. Boldini; A. Corradi; M. N. Losio; P. Bergonzini
• Keywords: Pseudorabies; Thymidine kinase; Strain (injury); Alphaherpesvirinae

Two mutant strains of pseudorabies virus (PRV) were selected from the virulent 86/27V treated with chemical drugs. The viruses, named 6A1 and 6C2, respectively, appeared to be unable express thymidine kinase function, as demonstrated by autoradiography test. They showed a reduced virulence for some susceptible animal species (chickens, mice, rabbits, calves, lambs piglets) was isolated sporadically. viruses able protect animals against infection strain PRV. At gross, well at histological examination, no lesions in apparatus, system tissues detected pigs inoculated 6C2 viruses. By contrast, rabbits presented lymphomononucleated cuffs, microgliosis, neuronophagia areas brain. This focal spreading, together absence neuronal necrosis intranuclear inclusions, suggest an induced modified