Isolation of triploid and tetraploid olive (Olea europaea L.) plants from mixoploid cv. ‘Frantoio’ and ‘Leccino’ mutants by in vivo and in vitro selection

• DOI: 10.1111/j.1439-0523.1996.tb00865.x
• Publication Date: 2006-04-30T05:33:09Z
• Authors: E. Rugini; G. Pannelli; M. Ceccarelli; M. Muganu
• Keywords: Olea; Oleaceae

Abstract This paper reports a procedure for isolating triploid and tetraploid olive plants, which have not been produced before either artificially or in nature. These polyploids were isolated from two mixoploid somatic mutants obtained earlier by treating Trantoio' ‘Leccino’ plantlets with gamma radiation. The exhibit increased thickness of leaf, stem root tissues, short internodes, modified leaf lamina shape strong resistance to Spilocaea oleagina (Cast.) Hugh. In addition, they produce mixture normal drupes some abnormally large ones, almost twice size. variation the nuclear DNA content is closely correlated their pollen size, crop capacity production fruit. Triploid genotypes 69 chromosomes germinating seeds these fruits, collected both mutants. Tetraploid plantlets, 92 chromosomes, cv. ‘Frantoio’ selecting vitro , during several proliferation phases, shoots obvate occurred among lanceolate intermediate shape.