Generation of marker‐free, bacterial blight‐resistant transgenic sterile line and hybrid rice with Xa21

• DOI: 10.1111/j.1439-0523.2011.01847.x
• Publication Date: 2011-02-15T13:05:10Z
• Authors: Lifen Gao; Zhihui Xia; Guanghuai Jiang; Hai Peng; Xianfeng Zhao; Wenxue Zhai
• Keywords: Genetically modified rice

With 6 figures and 4 tables Abstract Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. ( Xoo ) is a major production constraint in commercial hybrid because most the parental lines used are susceptible to . In this study, cloned BB resistance gene Xa21 was transferred into ‘D62B’, maintainer line three‐line indica using double right‐border vector pDRBXa21 through an Agrobacterium tumefaciens‐ mediated system. Molecular analysis revealed that integrated genome transgenic homozygous, single‐copy marker‐free D62B‐Xa21 obtained T 2 generation. then backcrossed with ‘D62A’ produce homozygous sterile D62A‐Xa21. The introduction did not cause any obvious alteration sterility other agronomic traits iodine–potassium iodide (I /KI) staining pollen grains field observation. Reverse transcriptase–polymerase chain reaction normal expression pattern lines. combinations derived from D62A‐Xa21 could inherit high BB.