Is Sperm Freezability Related to the Post‐Thaw Lipid Peroxidation and the Formation of Reactive Oxygen Species in Boars?

• DOI: 10.1111/j.1439-0531.2012.02126.x
• Publication Date: 2012-06-09T06:21:15Z
• Authors: J Gómez‐Fernández; E Gómez‐Izquierdo; C Tomás; E Mocé; E de Mercado
• Keywords: Cryopreservation; Male; 03 medical and health sciences; 0302 clinical medicine; Swine; Freezing; Animals; Lipid Peroxidation; Reactive Oxygen Species; Semen Preservation

The aim of the present study was to determine whether levels reactive oxygen species (ROS) substances production and lipid peroxidation sperm membrane were related quality that ejaculates exhibited after cryopreservation in boars. Ejaculates from 42 healthy boars used this they cryopreserved with lactose-egg yolk extender (LEY). Several parameters assessed by flow cytometry samples incubated for 30 150 min at 37 °C thawing: percentage intact plasma (SIPM), intracellular through mean DCF fluorescence intensity total (mean-DCF) viable non-viable containing oxidized BODIPY (VSOB NVSOB). In addition, percentages motile (TMS) progressively (PMS) same incubation times a computer-assisted analysis system. classification into good or bad freezers performed hierarchical cluster SIPM TMS post-thawing. those males classified as higher (p < 0.05) SPIM, PMS than freezers, although both groups presented similar > VSOB, NVSOB mean-DCF. Therefore, these results show amount are between freezers.