Colloidal Centrifugation of Stallion Semen Results in a Reduced Rate of Sperm DNA Fragmentation

• DOI: 10.1111/j.1439-0531.2012.02140.x
• Publication Date: 2012-07-07T07:07:40Z
• Authors: F Crespo; L Gutiérrez‐Cepeda; J Gosalvez; C Serres; SD Johnston
• Keywords: Fragmentation

Contents Stallion spermatozoa recovered and examined immediately after colloidal centrifugation resulted in a higher straight‐line velocity (VSL) than sperm processed using direct conventional (p = 0.000), but there was no differences the progressive motility or DNA fragmentation (SDF) as determined by chromatin dispersion assay. However, when centrifuged were incubated at 37°C for 24 h to determine rate of SDF (r‐SDF), lower r‐SDF 0.0011) observed those separation (0.5 ± 0.1%/h) compared (1.2 0.4%/h) (r‐SDF 1.2 0.3%/h). These results confirm that stallion prolonged longevity, these only apparent following period incubation dynamic assessment. Consequently, we strongly recommend use form assay evaluating and/or other ex vivo procedures, single basal assessment may inadvertently result false‐positive evaluation quality.