Ion‐channel currents of smooth muscle cells isolated from the prostate of guinea‐pig

• DOI: 10.1111/j.1464-410x.2003.04510.x
• Publication Date: 2003-11-24T22:13:37Z
• Authors: S.‐J. Oh; K.M. Kim; Y.‐S. Chung; E.‐K. Hong; S.Y. Shin; S.J. Kim
• Keywords: Tetraethylammonium; Apamin; Iberiotoxin; EGTA

OBJECTIVE To characterize the voltage‐activated ion‐channel currents in guinea‐pig prostate smooth muscle cells (GPSMCs). MATERIALS AND METHODS GPSMCs were isolated using collagenase, and used a whole‐cell patch clamp study. RESULTS When dialysed with CsCl solution all outward K + blocked step‐like depolarization (holding voltage − 70 mV) of cell membrane evoked inward that completely by nifedipine (1 µmol/L). With KCl solution, step depolarizations showed composed fast, transient current (I to ) did not inactivate. I was resistant high concentration tetraethylammonium (TEA, 5 mmol/L) but 4‐aminopyridine (5 mmol/L). The half‐activation half‐inactivation voltages 6 mV 58 mV, respectively. low Ca 2+ buffer (0.1 mmol/L EGTA) there spontaneous (STOCs) at depolarized (0 mV). STOCs TEA or iberiotoxin (10 nmol/L) insensitive apamin (100 nmol/L). CONCLUSION This voltage‐clamp study have l ‐type channels more than two types channels. voltage‐ time‐dependent changes these ion their interactions might be important forming action potentials regulating contractility.