Effects of potassium channel modulators on myogenic spontaneous phasic contractile activity in human detrusor from neurogenic patients

• DOI: 10.1111/j.1464-410x.2010.09935.x
• Publication Date: 2010-12-16T11:53:28Z
• Authors: Stephanie Oger; Delphine Behr‐Rou...; Diane Gorny; Jacques Bernabé; Eva Comperat; Emmanuel Chartier...; Pierre Denys; Francois Giuliano
• Keywords: Iberiotoxin; Pinacidil; Detrusor muscle; Apamin

To characterize the spontaneous contractile activity (SCA) developed by detrusor from patients with neurogenic overactivity (NDO) because alteration of properties plays a critical role in pathogenesis overactivity, as well to evaluate K(ATP) and K(Ca) channels on this SCA these regulate many species, including humans without overactive bladder (OAB).Human samples were obtained 44 undergoing cystectomy for cancer no known OAB symptoms 38 suffering urodynamically diagnosed NDO. Detrusor strips or urothelium/suburothelium mounted isometrically organ baths filled Krebs-HEPES (37 °C; 95% O(2) /5% CO(2) ). Strips incubated 10 µm pinacidil (K(ATP) opener) followed glibenclamide blocker). In another set experiments, 30 NS-1619 (BK(Ca) 100 nm iberiotoxin blocker) apamin (SK(Ca) blocker).SCA occurred more frequently larger amplitude area under curve NDO compared control patients. The presence did not significantly modify either patient population. Pinacidil markedly inhibited This effect was reversed glibenclamide. By contrast, elicit any significant changes patients, contrary patients.K(ATP) SK(Ca) patients' strips. BK(Ca) are involved regulation whereas they These results should be considered development K(+) openers treatment Moreover, observed vitro regarded an modelling human