A new autologous keratinocyte dressing treatment for non‐healing diabetic neuropathic foot ulcers

• DOI: 10.1111/j.1464-5491.2004.01166.x
• Publication Date: 2004-11-15T12:33:40Z
• Authors: M. Moustafa; C. Simpson; M. Glover; R. A. Dawson; S. Tesfaye; F. M. Creagh; D. Haddow; R. Short; S. Heller; S. MacNeil
• Keywords: Diabetic ulcers; Diabetic foot ulcer; Debridement (dental)

Abstract Aims To evaluate the use of a new cell‐tailored carrier surface (TranCell) for delivery autologous keratinocytes to promote wound healing in patients with chronic neuropathic foot ulcers. Methods TranCell is sterile medical grade polymer coated plasma‐polymerized functional containing 20% carboxylic acid which enables attach and proliferate. Six diabetic ulcers resistant conventional therapy were treated weekly applications delivered on TranCell. A split‐thickness skin biopsy was taken from each patient followed by isolation, expansion freezing down keratinocytes. Keratinocytes thawed seeded 48 h prior application. This procedure repeated addition until achieved. Results Complete achieved six out nine patients, reduction ulcer size one no response seen ulcer. Treatment discontinued due development Methicillin‐Resistant Staphylococcus aureus (MRSA) after only three Wound took 6–17 over 6–20 weeks. There recurrences healed follow‐up 6 months. Conclusions cells promising treatment side‐effects recurrence