Prevention of allergic asthma by vaccination with transgenic rice seed expressing mite allergen: induction of allergen‐specific oral tolerance without bystander suppression

• DOI: 10.1111/j.1467-7652.2011.00613.x
• Publication Date: 2011-03-30T02:27:15Z
• Authors: Kazuya Suzuki; Osamu Kaminuma; Lijun Yang; Toshiro Takai; Akio Mori; Makiko Umezu‐Goto; Takayuki Ohtomo; Yasushi Ohmachi; Yuko Noda; Sakiko Hirose; Ko Okumura; Hideoki Ogawa; Kazuko Takada; Masatomo Hirasawa; Takachika Hiroi; Fumio Takaiwa
• Keywords: Immunity, Cellular; Mice, Inbred BALB C; 0303 health sciences; Epitopes, T-Lymphocyte; Oryza; Bystander Effect; Immunoglobulin E; Plants, Genetically Modified; Asthma; 3. Good health; Arthropod Proteins; Cysteine Endopeptidases; Mice; 03 medical and health sciences; Desensitization, Immunologic; Antibody Formation; Animals; Humans; Female; Antigens, Dermatophagoides; Lung; Cell Proliferation; Plasmids

Summary This study tested the feasibility of oral immunotherapy for bronchial asthma using a newly developed subunit vaccine in which fragment (p45–145) mite allergen (Der p 1) containing immunodominant human and mouse T cell epitopes was encapsulated endoplasmic reticulum‐derived protein bodies transgenic (Tg) rice seed. Allergen‐specific serum immunoglobulin responses, proliferation, Th1/Th2 cytokine production, airway inflammatory infiltration, hyper‐responsiveness (BHR) lung histology were investigated allergen‐immunized ‐challenged mice. Prophylactic vaccination with Tg seeds clearly reduced levels allergen‐specific IgE IgG. Allergen‐induced CD4 + proliferation production Th2 cytokines vitro , infiltration eosinophils, neutrophils mononuclear cells into airways BHR also inhibited by vaccination. The effects antigen‐specific immune response because specific IgG mice immunized Der f 2 or ovalbumin not significantly suppressed 1 expressing rice. Thus, does induce nonspecific bystander suppression, has been problem many tolerance regimens. These results suggest that our novel strategy is promising approach against allergic diseases including asthma.