EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST)

• DOI: 10.1111/j.1469-0691.2012.03880.x
• Publication Date: 2012-05-01T16:37:24Z
• Authors: Maiken C. Arendrup; Manuel Cuenca-Est...; Cornelia Lass-Flörl; William Hope
• Keywords: Microbiology (medical); 0301 basic medicine; Antifungal Agents; Time Factors; susceptibility testing; Microbial Sensitivity Tests; minimum inhibitory concentration; yeast; Europe; Cryptococcus; 03 medical and health sciences; Infectious Diseases; Yeasts; Freezing; Solvents; Humans; EUCAST; N4i 2: Invasive mycoses and compromised host NCMLS 1: Infection and autoimmunity; antifungals; Candida

The European Committee on Antimicrobial Susceptibility Testing-Subcommittee on Antifungal Susceptibility Testing (EUCAST-AFST) has revised the EDef 7.1 document on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for fermentative yeasts. Changes are: dimethylsulphoxide is now the recommended solvent for caspofungin, micafungin and fluconazole; the shelf-life of plates containing the echinocandins prepared from stock solutions in dimethylsulphoxide is extended to 6 months at -80°C; testing of amphotericin and Cryptococcus has been incorporated; and minimum inhibitory concentration ranges for quality control strains and anidulafungin are included.