Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice

• DOI: 10.1111/j.1469-7793.2000.00771.x
• Publication Date: 2004-08-13T08:57:08Z
• Authors: Yuanlin Song; Norimasa Fukuda; Chunxue Bai; Tonghui Ma; Michael A. Matthay; A. S. Verkman
• Keywords: Aquaporin 1; Amiloride

1 Aquaporin (AQP) water channels provide a major pathway for osmotically driven movement across epithelial and microvascular barriers in the lung. We used mice deficient each of three principal lung aquaporins, AQP1, AQP4 AQP5, to test hypothesis that aquaporins are important neonatal fluid balance, adult clearance formation oedema after acute injury. 2 Wet-to-dry weight ratios (W/D) lungs from wild-type decreased 7.9 5.7 over first hour spontaneous delivery. AQP deletion did not significantly affect W/D at 45 min birth. 3 Alveolar was measured living ventilated which 0.5 ml saline containing radiolabelled albumin instilled into airspaces. Fluid 17.4% 15 inhibited >90% by amiloride, but affected deletion. 4 established models injury – acid aspiration thiourea administration. Two hours intratracheal administration HCl, increased 3.7 7.5 Three intraperitoneal infusion thiourea, 5.5 marked pleural effusions appeared, there were no differences knockout mice. 5 Hyperoxic subacute induced 95% oxygen. Neither mean survival (143 h) nor 65 h (5.1) 6 Despite their role transport, required physiological or lung, accumulation extravascular injured