Compartmental models of rat cerebellar Purkinje cells based on simultaneous somatic and dendritic patch‐clamp recordings

• DOI: 10.1111/j.1469-7793.2001.00445.x
• Publication Date: 2004-08-05T09:27:31Z
• Authors: Arnd Roth; Michael Häusser
• Keywords: 6-Cyano-7-nitroquinoxaline-2,3-dione; 0301 basic medicine; Patch-Clamp Techniques; Excitatory Postsynaptic Potentials; Tetraethylammonium; Dendrites; Tetrodotoxin; Cell Compartmentation; Membrane Potentials; Rats; GABA Antagonists; Pyridazines; Purkinje Cells; 03 medical and health sciences; Cadmium Chloride; Cations; Potassium Channel Blockers; Animals; 4-Aminopyridine; Rats, Wistar; Excitatory Amino Acid Antagonists; Cell Size

1 Simultaneous dendritic and somatic patch-clamp recordings were made from Purkinje cells in cerebellar slices 12- to 21-day-old rats. Voltage responses current impulses injected via either the or pipette obtained presence of selective Ih blocker ZD 7288 blockers spontaneous synaptic input. Neurons filled with biocytin for subsequent morphological reconstruction. 2 Four neurons reconstructed converted into detailed compartmental models. The specific membrane capacitance (Cm), resistance (Rm) intracellular resistivity (Ri) optimized by direct fitting model electrophysiological data same cell. Mean values were: Cm, 0.77 ± 0.17 μF cm−2 (mean ±s.d.; range, 0.64-1.00 cm−2), Rm, 122 18 kΩ cm2 (98-141 cm2) Ri, 115 20 Ω cm (93-142 cm). 3 steady-state electrotonic architecture these was compact under experimental conditions used. However, voltage-clamp parallel fibre climbing currents substantially filtered attenuated. 4 models compared a two-compartment cells. range kinetics that can be faithfully recorded using voltage clamp is predicted fairly well model, even though some its underlying assumptions are violated. 5 A constructed based on data, inserted passive Somatic EPSP amplitude attenuated EPSPs at their site generation. efficacy quantal conductance, as measured amplitude, only weakly dependent location spiny branchlets. 6 structure unusual very compact, while transients such potentials action heavily filtered.