Only an early nitric oxide burst and the following wave of secondary nitric oxide generation enhanced effective defence responses of pelargonium to a necrotrophic pathogen

• DOI: 10.1111/j.1469-8137.2007.02142.x
• Publication Date: 2007-07-04T15:59:24Z
• Authors: Jolanta Floryszak...; Magdalena Arasimo...; Grzegorz Milczarek; Henryk Jelen; Hanna Jackowiak
• Keywords: Pelargonium

Participation of nitric oxide (NO) in cross-talk between ivy pelargonium (Pelargonium peltatum) leaves and Botrytis cinerea was investigated using electrochemical biochemical approaches. In response to the necrotroph, initiated a near-immediate NO burst, but specificity its generation dependent on genetic makeup host plant. resistant cultivar, strong burst followed by wave secondary generation, shown bio-imaging with DAF-2DA. The epicentre synthesis located targeted cells, which exhibited TUNEL-positive reaction. Soon after challenge, an elevated concentration hydrogen peroxide (H(2)O(2)) correlated reversible inhibition catalase (CAT), ascorbate peroxidase (APX), suppression ethylene synthesis. induced initially expanded then gradually disappeared successive days, provoking noncell-death-associated resistance enhanced pool antioxidants, finally favoured maintenance homeostasis surrounding cells. By contrast, susceptible pelargonium, weak recorded further increased only as disease progressed, accompanied very intensive H(2)O(2) pathogen colonizing cells also acquired ability produce considerable amounts nitrosative oxidative stress tissues.