Carbon monoxide alleviates cadmium‐induced oxidative damage by modulating glutathione metabolism in the roots of Medicago sativa

• DOI: 10.1111/j.1469-8137.2007.02251.x
• Publication Date: 2007-11-20T17:38:38Z
• Authors: Yi Han; Jing Zhang; Xiaoyue Chen; Zhaozhou Gao; Wei Xuan; Sheng Xu; Xiao Ding; Wenbiao Shen
• Keywords: Glutathione disulfide

• Using pharmacological and biochemical approaches, the role of cadmium (Cd)-induced carbon monoxide (CO) release relationship between CO oxidative stress conferred by Cd exposure in root tissues alfalfa (Medicago sativa) plants were investigated. treatments showed a dose-dependent enhancement lipid peroxidation. Both 100 200 µm CdCl2 caused increase release, which is consistent with changes activity synthetic enzyme heme oxygenase (HO) its HO-1 transcript. A enhanced formation nonprotein thiols (NPT), reduced glutathione (GSH) to oxidized (GSSG), was potentiated pretreatment scavenger hemoglobin (Hb). Plants pretreated for 6 h 50% CO-saturated aqueous solution, induced rapid endogenous followed gradual decrease when subsequently exposed 72 h, effectively decreased damage. Meanwhile, modulated several enzymes responsible GSH metabolism, thus resulting partial restoration : GSSG ratio, significantly blocked Hb. These results are suggestive as signal element alleviation Cd-induced damage modulating metabolism.