An experimental method to facilitate the identification of hybrid sporophytes in the moss Physcomitrella patens using fluorescent tagged lines

• DOI: 10.1111/j.1469-8137.2011.03668.x
• Publication Date: 2011-03-03T04:43:40Z
• Authors: Pierre‐François P...; David J. Cove; Ralph S. Quatrano; Stuart F. McDaniel
• Keywords: Physcomitrella patens; Sporophyte; Outcrossing; Reverse Genetics

• The sequencing of the Physcomitrella patens genome, combined with high frequency gene targeting in this species, makes it ideal for reverse genetic studies. For forward studies, experimental crosses and analysis progeny are essential. Since P. is monoicous, producing both male female gametes on same gametophore, undergoing self-fertilization at a frequency, identification crossed sporophytes difficult. Usually spores from many mixed culture must be tested production recombinant progeny. Here, we describe use transgenic lines that express fluorescent transgene constitutively, to provide direct visual screen hybrid sporophytes. We show segregations obtained technique as expected, demonstrate its utility study rate outcrossing between three isolates patens.