Heterotypic and homotypic cell‐cell adhesion molecules in endothelial cells

• DOI: 10.1111/j.1470-8744.1990.tb00132.x
• Publication Date: 2023-10-23T01:38:53Z
• Authors: VK Kalra; R. Banerjee; N. Sorgente
• Keywords: Molecular Sequence Data; Antibodies, Monoclonal; Erythrocytes, Abnormal; Platelet Membrane Glycoproteins; Plasma; Biopolymers; Intercellular Junctions; Microscopy, Fluorescence; Antigens, Surface; Liposomes; Cell Adhesion; Animals; Humans; Cattle; Amino Acid Sequence; Endothelium, Vascular; Cell Adhesion Molecules; Oligopeptides; Cells, Cultured; Phospholipids

Sickle red blood cells display an abnormal propensity to adhere cultured bovine aortic endothelial when compared normal cells. The adherence was potentiated three‐fold by cell derived conditioned medium, enriched in multimers of von Willebrand factor. Such ablated 80% either the synthetic peptide (RGDS) or antibody GPIIb/IIIa, indicating presence RGD recognition domain/receptor sickle both. also inhibited 70% phosphatidylserine, but not other phospholipids, putative receptors for this phospholipid labeling with monoclonal antibodies revealed localization MAB D2 regions cell‐cell contact. antigen on which cross‐reacts has a Mr 130,000. addition such during plating disrupted monolayer formation. It appears that 130‐kDa polypeptide is recognized D2, may be adhesion molecule.