Induction of secretory phospholipase A2 in reactive astrocytes in response to transient focal cerebral ischemia in the rat brain

• DOI: 10.1111/j.1471-4159.2004.02540.x
• Publication Date: 2004-07-16T08:31:03Z
• Authors: Teng‐Nan Lin; Qun Wang; Agnes Simonyi; Jean‐Ju Chen; Wai‐Mui Cheung; Yong Y. He; Jan Xu; Albert Y. Sun; Chung Y. Hsu; Grace Y. Sun
• Keywords: Northern blot; Neuroglia

Abstract Although mRNA expression of group IIA secretory phospholipase A 2 (sPLA ‐IIA) has been implicated in responses to injury the CNS, information on protein remains unclear. In this study, we investigated temporal and spatial sPLA ‐IIA immunoreactivity transient focal cerebral ischemia induced rats by occlusion middle artery. Northern blot analysis showed a biphasic increase following 60‐min ischemia–reperfusion: an early phase at 30 min second late ranging from 12 h 14 days. situ hybridization localized early‐phase affected ischemic cortex late‐phase penumbral area. Besides mRNA, glial fibrillary acidic (GFAP) cyclo‐oxygenase‐2 mRNAs, but not cytosolic PLA , also area 3 days after ischemia–reperfusion. Immunohistochemistry indicated positive cells similar GFAP‐positive astrocytes different isolectin B4‐positive microglial cells. Confocal microscopy further confirmed reactive Taken together, these results demonstrate for first time up‐regulation inflammatory response