Induction of glial glutamate transporters in adult mesenchymal stem cells

• DOI: 10.1111/j.1471-4159.2004.02709.x
• Publication Date: 2006-03-03T12:16:14Z
• Authors: Isabelle De Hempt...; Céline Vermeiren; Jean‐Marie Maloteaux; Emmanuel Hermans
• Keywords: Nestin; Neuroepithelial cell

Abstract Adult bone marrow mesenchymal stem cells are multipotent that can differentiate into a variety of mesodermal tissues. Recent studies have reported on their ability to also evolve non‐mesodermal cells, especially neural cells. While most these revealed manipulating triggers the expression typical neurone markers, less is known about induction neuronal‐ or glial‐related physiological properties. The present study focused characterisation glutamate transporters and activity in rat grown culture conditions favouring differentiation astroglial Ten days exposure supplement G5 was found increase nestin (neuro‐epithelial cell intermediate filament), an filament protein expressed by Simultaneously, robust high‐affinity transporter GLT‐1 (and GLAST) detected RT‐PCR immunocytochemistry. This correlated with highly significant Na + ‐dependent [ 3 H] d ‐aspartate uptake. Finally, while glial fibrillary acidic immunoreactivity could not be detected, induced astrocytic enzyme glutamine synthetase demonstrated. These results indicate vitro adult precursors coincides functional transport systems. Although nature remains confirmed, this observation gives support as promising tool for treatment neurological diseases involving excitoxicity.