Ginkgo biloba affords dose‐dependent protection against 6‐hydroxydopamine‐induced parkinsonism in rats: neurobehavioural, neurochemical and immunohistochemical evidences

• DOI: 10.1111/j.1471-4159.2005.03000.x
• Publication Date: 2005-03-09T23:59:18Z
• Authors: Muzamil Ahmad; Sofiyan Saleem; Abdullah Shafique...; Seema Yousuf; Mubeen Ahmad Ansari; M Badruzzaman Khan; Tauheed Ishrat; Rajnish Kumar Cha...; Ashok Kumar Agrawal; Fakhrul Islam
• Keywords: TBARS; Hydroxydopamine; Neurochemical; Glutathione reductase

Abstract Ginkgo biloba extract (EGb), a potent antioxidant and monoamine oxidase B (MAO‐B) inhibitor, was evaluated for its anti‐parkinsonian effects in 6‐hydroxydopamine (6‐OHDA) rat model of the disease. Rats were treated with 50, 100, 150 mg/kg EGb 3 weeks. On day 21, 2 µL 6‐OHDA (10 µg 0.1% ascorbic acid saline) injected into right striatum, while sham‐operated group received vehicle. Three weeks after injection, rats tested rotational behaviour, locomotor activity, muscular coordination. After 6 weeks, they killed to estimate generation thiobarbituric reactive substances (TBARS) reduced glutathione (GSH) content, measure activities glutathione‐ S ‐transferase (GST), reductase (GR), peroxidase (GPx), catalase, superoxide dismutase (SOD), quantify catecholamines, dopamine (DA) D2 receptor binding, tyrosine hydroxylase‐immunoreactive (TH‐IR) fibre density. The increase drug‐induced rotations deficits activity coordination due injections significantly dose‐dependently restored by EGb. lesion followed an increased TBARS significant depletion GSH content substantia nigra, which gradually treatment. also glutathione‐dependent enzymes, SOD had lesioning. A decrease level DA metabolites number dopaminergic receptors striatum observed both recovered following Finally, all these results exhibited density TH‐IR fibers ipsilateral nigra lesioned treatment EGb; lesioning induced almost complete loss fibers. Considering our behavioural studies, biochemical analysis, immunohistochemical observation, we conclude that can be used as therapeutic approach check neuronal parkinsonism.