Proteomic analysis of rat hippocampal plasma membrane: characterization of potential neuronal‐specific plasma membrane proteins

• DOI: 10.1111/j.1471-4159.2006.03934.x
• Publication Date: 2006-06-26T16:14:28Z
• Authors: Ping Chen; Xuanwen Li; Yalan Sun; Zhen Liu; Rui Cao; Quanyuan He; Meichi Wang; Jixian Xiong; Jinyun Xie; Xianchun Wang; Songping Liang
• Keywords: Differential centrifugation

Abstract The hippocampus is a distinct brain structure that crucial in memory storage and retrieval. To identify comprehensively proteins of hippocampal plasma membrane (PM) detect the neuronal‐specific PM proteins, we performed proteomic analysis rat using following three technical strategies. First, were purified by differential density‐gradient centrifugation from tissue separated one‐dimensional electophoresis, digested with trypsin analyzed electrospray ionization (ESI) quadrupole time‐of‐flight (Q‐TOF) tandem mass spectrometry (MS/MS). Second, tryptic peptide mixture PMs method was liquid chromatography ion‐trap ESI‐MS/MS. Finally, primary neurons biotin‐directed affinity technique electrophoresis, ESI‐Q‐TOF‐MS/MS. A total 345, 452 336 non‐redundant identified each procedure respectively. There 867 protein entries, which 64.9% are integral or membrane‐associated proteins. One hundred eighty‐one detected only could be regarded as neuronal marker candidates. We also found some hypothetical no functional annotations first PM. This work will pave way for further elucidation mechanisms function.