AKAP79/150 interacts with the neuronal calcium‐binding protein caldendrin

• DOI: 10.1111/j.1471-4159.2012.07828.x
• Publication Date: 2012-06-13T16:18:08Z
• Authors: Xenia Gorny; Marina Mikhaylova; Christian Seeger; Pasham Parameshwa...; Carsten Reissner; Björn H. Schott; U. Helena Danielson; Michael R. Kreutz; Constanze Seidenb...
• Keywords: Postsynaptic density; Dephosphorylation; PDZ domain; Interactome

The A kinase-anchoring protein AKAP79/150 is a postsynaptic scaffold molecule and key regulator of signaling events. At the postsynapse it coordinates phosphorylation dephosphorylation receptors via anchoring kinases phosphatases near their substrates. Interactions between AKAP79 two Ca(2+) -binding proteins caldendrin calmodulin have been investigated here. Calmodulin known interaction partner that has shown to regulate activity kinase PKC in -dependent manner. Pull-down experiments surface plasmon resonance biosensor analyses used here demonstrate can also interact with caldendrin, neuronal calcium-binding implicated regulation -influx release. We compete for partially overlapping binding site on differentially dependent calcium. Therefore, this competition regulated by calcium levels. Moreover, both different characteristics suggesting might play complementary roles. enrichment, complex mechanism, calmodulin, makes an interesting novel player toolkit AKAP interactome.