Huntingtin associated protein 1 regulates trafficking of the amyloid precursor protein and modulates amyloid beta levels in neurons

• DOI: 10.1111/j.1471-4159.2012.07845.x
• Publication Date: 2012-06-25T19:31:49Z
• Authors: Gui‐Zhi Yang; Miao Yang; Yoon Lim; Jian‐Jun Lu; Ting‐Hua Wang; Jian‐Guo Qi; Jin‐Hua Zhong; Xin‐Fu Zhou
• Keywords: Amyloid (mycology); BETA (programming language); Amyloid beta

J. Neurochem . (2012) 122 , 1010–1022. Abstract Amyloid precursor protein (APP) is involved in the pathogenesis of Alzheimer’s disease. It axonally transported, endocytosed and sorted to different cellular compartments where amyloid beta (Aβ) produced. However, mechanism APP trafficking remains unclear. We present evidence that huntingtin associated 1 (HAP1) may reduce Aβ production by regulating non‐amyloidogenic pathway. HAP1 are highly colocalized a number brain regions, with similar distribution patterns both mouse human brains. They each other, interacting site 371–599 HAP1. more retained cis‐Golgi, trans‐Golgi complex, early endosome ER‐Golgi intermediate compartment HAP1−/− neurons. deletion significantly alters endocytosis reduces re‐insertion into cytoplasmic membrane. protein‐YFP(APP‐YFP) vesicles neurons reveal decreased rate an increased motionless vesicles. Knock‐down cultured cortical disease model increases levels. Our data suggest regulates subcellular pathway negatively regulate