Near‐complete adaptation of the PRiMA knockout to the lack of central acetylcholinesterase

• DOI: 10.1111/j.1471-4159.2012.07856.x
• Publication Date: 2012-07-02T20:54:02Z
• Authors: Vladimir Farar; Franziska Mohr; Marie Legrand; Boris Lamotte d’I...; Jan Cendelin; Jacqueline Leroy; Marc Abitbol; Veronique Bernard; Frédéric Baud; Vincent Fournet; Pascal Houze; Jochen Klein; Benoit Plaud; Jan Tuma; Martina Zimmermann; Philippe Ascher; Anna Hrabovska; Jaromir Myslivecek; Eric Krejci
• Keywords: 0301 basic medicine; Pyridines; Microdialysis; Cholinergic Agents; Muscle Proteins; Membrane Potentials; Body Temperature; Choline; Mice; Receptors; Scopolamine Hydrobromide; Gait; Neurons; Ingrown; Heterocyclic; Statistics; Brain; Adaptation, Physiological; Neostigmine; 3. Good health; Spinal Cord; Acetylcholinesterase; [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]; Collagen; Drug; Protein Binding; Physiological; Knockout; 610; Nerve Tissue Proteins; Muscarinic Antagonists; Motor Activity; In Vitro Techniques; Tritium; Dose-Response Relationship; Bridged Bicyclo Compounds; 03 medical and health sciences; Muscarinic; Animals; Nonparametric; [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]; Adaptation; Maze Learning; Radioisotopes; Dose-Response Relationship, Drug; Membrane Proteins; Pirenzepine; Dihydro-beta-Erythroidine; Newborn; Bridged Bicyclo Compounds, Heterocyclic; Bungarotoxins; Acetylcholine; Nails; Animals, Newborn; Gene Expression Regulation; Rotarod Performance Test; Exploratory Behavior; Cholinesterase Inhibitors

Acetylcholinesterase (AChE) rapidly hydrolyzes acetylcholine. At the neuromuscular junction, AChE is mainly anchored in extracellular matrix by collagen Q, whereas brain, tethered proline-rich membrane anchor (PRiMA). The AChE-deficient mice, which has been deleted from all tissues, have severe handicaps. Surprisingly, PRiMA KO mice mostly eliminated brain show very few deficits. We now report that most of changes observed and particular high levels ambient acetylcholine massive decrease muscarinic receptors, are also KO. However, two groups mutants differ their responses to inhibitors. Since PRiMA-KO similar low concentrations but content peripheral nervous system, these results suggest system a major target inhibitors, its absence AChE- deficient main cause slow development vulnerability mice. level adaptation nearly complete.