Development of RAPD protocol for typing of strains of lactic acid bacteria and enterococci

• DOI: 10.1111/j.1472-765x.1995.tb01085.x
• Publication Date: 2008-03-19T16:31:02Z
• Authors: P.S. Cocconcelli; D. Porro; S. Galandini; L. Senini
• Keywords: Enterococcus faecium; Enterococcus faecalis; Lactobacillus reuteri; Primer (cosmetics); Lactobacillus helveticus

P.S. COCCONCELLI, D. PORRO, S. GALANDINI AND L. SENINI. 1995. A protocol for typing strains of lactic acid bacteria and enterococci based on randomly amplified polymorphic DNA (RAPD) fragments has been developed. Using a single 10-mer primer, fingerprints were achieved without the need to isolate genomic DNA. Different conditions release amplification investigated in order obtain reproducible results high discrimination among strains. This RAPD was successfully applied belonging species Lactobacillus acidophilus, Lact. helveticus, casei, reuteri, plantarum, Enterococcus faecalis, Ent. faecium Streptococcus thermophilus.