Identification and pathogenicity of Vibrio ponticus affecting cultured Japanese sea bass, Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes)

• DOI: 10.1111/j.1472-765x.2007.02141.x
• Publication Date: 2007-06-16T13:30:36Z
• Authors: Z.Y. Xie; C.Q. Hu; L.P. Zhang; C. Chen; C.H. Ren; Q. Shen
• Keywords: Lateolabrax; Sea bass; Perciformes; Marine fish

To rapidly determine the causative agent of mass death in Lateolabrax japonicus Zhelin Bay Guangdong Province China April 2004.Thirty-six strains, numbered sequentially from RP01 to RP36, were isolated six diseased fish. All strains identified as being same vibrio species according results universal primer PCR combined with DGGE (UPPCR-DGGE). RP30 was one these that randomly selected and analysed by using a morphological, physiological biochemical plate, Biolog GN2 Microplate System API 20E system. Furthermore, RP30' 16S rDNA sequenced aligned Genbank. Its virulence (Cuvier Cuvier Valenciennes) also tested. is most closely related four Vibrio ponticus (99.3% similarity). LD50s 2.5 (x103 CFU per fish for intraperitoneal inoculation (IP) 3.2 intramuscular (IM), respectively.The investigated pathogenic V. ponticus.UPPCR-DGGE very helpful epidemiologic investigation. Interestingly, this first report infects cultured marine likewise introduced investigation disease.