A novel real-time PCR-based method for the detection of Listeria monocytogenes in food

• DOI: 10.1111/j.1472-765x.2007.02234.x
• Publication Date: 2007-10-24T04:19:36Z
• Authors: K. Oravcová; T. Kuchta; E. Kaclíková
• Keywords: Food microbiology

A new real-time PCR-based method was developed for the detection of Listeria monocytogenes in food.A two-step enrichment involving a 24-h incubation half-Fraser broth followed by 6-h subculture Fraser used, cell lysis and PCR with primers TaqMan probe previously our laboratory. When evaluated 144 naturally contaminated food samples, 44 were detected as positive 42 standard EN ISO 11290-1. With 61 samples artificially at level 10(0) CFU per 25 g, 58 respective methods.The facilitated L. on next day after sample reception, reduction false-positive results because dead bacterial cells false-negative inhibitors.The can be used faster alternative to current methods.