Involvement of protein phosphatases in differential regulation of renal proximal tubular PAH and sodium‐dependent dicarboxylate transport*

• DOI: 10.1111/j.1472-8206.2000.tb00433.x
• Publication Date: 2009-08-26T18:43:54Z
• Authors: G. Gabriëls; S. Mauß; A. Werners; J. Greven
• Keywords: Dephosphorylation

Abstract— It has been demonstrated that the basolateral organic anion (PAH) transporter and sodium‐dependent dicarboxylate of rabbit renal proximal tubules are regulated differentially. A variety protein kinases shown to be involved in regulation transport while uptake, which first is coupled functionally, not inlluenced by these kinases. This study was undertaken elucidate whether respective activities modulated differentially phosphatases as well. The experiments were performed on isolated S 2 segments microdissected from kidneys without use enzymatic agents. 3 H‐PAH used marker substance PAH transporter. 14 C‐glularate a sodium/dicarboxylate cotransporter. 30 s tubular uptake measurements performed. Vanadate (10 −3 M). selective inhibitor tyrosine phosphatase, did reduce significantly, inhibitors serine/threonine I 2A. okadaic acid calyculin 6 M. each) induced significant decrease (by 32.3% ± 7.9% 25.6% 6.4%) but change transport. These findings indicate that, addition kinases, have role There no effect 30s glularate Thus, additional evidence for differential short‐time activity transporters dicarboxylales provided.