Antifibrotic and fibrolytic properties of celecoxib in liver damage induced by carbon tetrachloride in the rat

• DOI: 10.1111/j.1478-3231.2010.02256.x
• Publication Date: 2010-05-27T06:49:36Z
• Authors: Enrique Chávez; José Segovia; Mineko Shibayama; Victor Tsutsumi; Paula Vergara; Luis Castro-Sánchez; Eduardo Pérez Sal...; Mario G. Moreno; Pablo Muriel
• Keywords: Celecoxib; Hepatic stellate cell; Hydroxyproline; CCL4

Background: Transforming growth factor-β (TGF-β) plays a pivotal role in liver fibrosis, because it activates hepatic stellate cells, stimulating extracellular matrix deposition. Cyclooxygenase-2 (COX-2) has been associated with TGF-β its inhibition decreases expression and collagen production some cultured cell types. Aim: The aim of this work was to evaluate the ability celecoxib (a selective COX-2 inhibitor) prevent reverse fibrosis induced by CCl4. Methods: We established experimental groups rats including vehicle drug controls, damage chronic CCl4 administration plus pharmacological treatment both prevention reversion models. determined: alanine aminotransferase, alkaline phosphatase, γ-glutamyl transpeptidase, COX metalloproteinase-2 -9 activities, lipid peroxidation, glutathione levels, glycogen content expression. Results: Celecoxib prevented aided recovery livers necrotic cholestatic damage. exhibited anti-oxidant properties restoring redox equilibrium (lipid peroxidation levels). Glycogen decreased CCl4, while partially reversed effect. inhibited activity, expression, activity and, consequently, accumulation. Conclusion: Our findings indicate that exerts strong antifibrogenic fibrolytic effects model cirrhosis.