TheYCR079wgene confers a rapamycin-resistant function and encodes the sixth type 2C protein phosphatase inSaccharomyces cerevisiae

• DOI: 10.1111/j.1567-1364.2006.00160.x
• Publication Date: 2006-09-27T09:17:42Z
• Authors: Haihua Ruan; Zhihui Yan; Hao Sun; Linghuo Jiang
• Keywords: Sirolimus; 0303 health sciences; 03 medical and health sciences; Antifungal Agents; Saccharomyces cerevisiae Proteins; Drug Resistance, Fungal; Genes, Fungal; Molecular Sequence Data; Phosphoprotein Phosphatases; Amino Acid Sequence; Saccharomyces cerevisiae; Gene Deletion

Type 2C protein phosphatase (PP2C) is a monomeric enzyme and requires Mg(2+) or Mn(2+) for its activity. Up to now, seven PP2C-like genes have been identified in the genome of Saccharomyces cerevisiae. However, the protein encoded by the sixth PP2C-like gene, YCR079w, has not been demonstrated to have PP2C activity. In this study, we show that YCR079w confers a rapamycin-resistant function in yeast cells, and we also demonstrate that the YCR079w-encoded protein exhibits characteristics of a typical PP2C. Therefore, YCR079w encodes the sixth PP2C, PTC6, in budding yeast.