Single QTL mapping and nucleotide-level resolution of a physiologic trait in wineSaccharomyces cerevisiaestrains

• DOI: 10.1111/j.1567-1364.2007.00252.x
• Publication Date: 2007-06-01T03:31:26Z
• Authors: Philippe Marullo; Michel Aigle; Marina Bely; Isabelle Masneuf-...; Pascal Durrens; Denis Dubourdieu; Gaël Yvert
• Keywords: Fermentation in winemaking

Natural Saccharomyces cerevisiae yeast strains exhibit very large genotypic and phenotypic diversity. However, the link between phenotype variation genetic determinism is still difficult to identify, especially in wild populations. Using genome hybridization on DNA microarrays, it now possible identify single-feature polymorphisms among divergent strains. This tool offers possibility of applying quantitative genetics In this instance, we studied basis for variations acetic acid production using progeny derived from two grape must isolates. The trait was quantified during alcoholic fermentation 108 segregants their crossing. A map 2212 markers generated oligonucleotide a major locus (QTL) mapped with high significance. Further investigations showed that QTL due nonsynonymous single-nucleotide polymorphism targeted catalytic core asparaginase type I (ASP1) abolished its activity. only effective when asparagine used as nitrogen source. Our results assimilation CO(2) rate production, well as, broader scale, illustrating specific problem working nonlaboratory microorganisms.