Interactions between ectomycorrhizal symbiosis and fluorescent pseudomonads on Acacia holosericea: isolation of mycorrhiza helper bacteria (MHB) from a Soudano-Sahelian soil

• DOI: 10.1111/j.1574-6941.2002.tb00964.x
• Publication Date: 2006-01-05T13:50:28Z
• Authors: Hassna Founoune; Robin Duponnois; Jean Marie Meyer; Jean Thioulouse; Dominique Masse; Jean Luc Chotte; Marc Neyra
• Keywords: Pisolithus

Acacia holosericea seedlings were planted in 1-l pots filled with a soil collected from an Australian plantation Southern Senegal. After 6 months of culture, mycorrhizosphere soil, roots, galls induced by root-knot nematodes and Rhizobium nodules sampled each pot. The diversity this bacterial group was characterized siderotyping (pyoverdine IsoElectric Focusing (IEF) analysis) restriction fragment length polymorphism (RFLP). effect these isolates on the establishment ectomycorrhizal symbiosis between (A. holosericea) Pisolithus sp. strain IR100 studied. In population fluorescent pseudomonads represented strains two different siderovars (groups presenting identical pyoverdine–IEF pattern): siderovar 1 (74%) 2 (26%). around revealed three (40% 1, 40% about 15% 3). RFLP 16S rDNA divided into four groups MspI, HhaI HaeIII endonucleases. A. promoted 14 isolated roots galls. Shoot biomass stimulated eight six seven roots. These mycorrhiza helper bacteria could have great ecological importance tropical areas through reforestation programs.