β-1,3-Glucan reduces growth ofMycobacterium tuberculosisin macrophage cultures

• DOI: 10.1111/j.1574-695x.2002.tb00570.x
• Publication Date: 2006-01-09T14:59:00Z
• Authors: Geir Hetland; Per Sandven
• Keywords: Antibody opsonization

The effect of β-1,3-d-glucans SSG, from Sclerotinia sclerotiorum, or soluble (sMG) particulate (pMG) MacroGard® baker's yeast on growth Mycobacterium tuberculosis H37Rv in cultures peritoneal macrophages BALB/c mice was examined. After 24 h intracellular bacteria lysed were cultured and the number cfu counted. SSG given with challenge, but not after, reduced M. significantly. pMG, sMG, challenge had an even stronger inhibitory effect, which enhanced after serum opsonization particles. serum-treated pMG abrogated by addition a monoclonal antibody to CD11b. results indicate that β-glucans inhibit host cells vitro, probably due cellular stimulation and/or competitive inhibition uptake via CR3 (CD11b/18).