Production of novel polyhydroxyalkanoates byPseudomonas stutzeri1317 from glucose and soybean oil

• DOI: 10.1111/j.1574-6968.1998.tb13297.x
• Publication Date: 2006-01-17T09:12:07Z
• Authors: Wennan He; Weidong Tian; Guang Zhang; Guo-Qiang Chen; Zengming Zhang
• Keywords: 0301 basic medicine; 03 medical and health sciences

A strain of Pseudomonas stutzeri coded 1317 isolated from oil-contaminated soil was found to grow well in glucose and soybean oil as a sole carbon source, respectively. Polyhydroxyalkanoates containing medium chain length monomers of hydroxyalkanoates ranging from C6 to C14 were synthesized up to 52% of cell dry weight of P. stutzri 1317 grown on glucose mineral media. In mineral media containing 10 g l−1 soybean oil, P. stutzeri 1317 produced up to 63% of polyhydroxyalkanoates containing mainly a novel monomer of 3,6-epoxy-7-nonene-1,9 dioic acids making up to 63% of the polyhydroxyalkanoates, together with minor monomers of C8 and C10. 1H- and 13C-nuclear magnetic resonance spectroscopy and electron impact gas chromatography-mass spectroscopy and chemical ionization gas chromatography-mass spectroscopy were used to study the structures of polyhydroxyalkanoates synthesized by P. stutzeri 1317.