Expression of green fluorescent protein inLactococcus lactis

• DOI: 10.1111/j.1574-6968.2000.tb08963.x
• Publication Date: 2006-01-10T07:51:59Z
• Authors: Pilar Fernández ...; Concha Nieto; Paloma Acebo; Manuel Espinosa; Paloma López
• Keywords: Aequorea victoria; Biovar

The gfp gene from Aequorea victoria, encoding the green fluorescent protein (GFP) has been expressed in Lactococcus lactis subsp. biovar cremoris MG1363, upon construction and introduction of plasmid pLS1GFP into this host. GFP was monitored living cells during growth to evaluate its use molecular physiological studies. Quantification levels by cultures feasible fluorescence spectroscopy. Phase-contrast microscopy allowed us distinguish, mixed cultures, lactococcal expressing GFP. Our results indicate that can be used as a reporter L. lactis.