Membrane vesicles released byActinobacillus pleuropneumoniaecontain proteases and Apx toxins

• DOI: 10.1111/j.1574-6968.2000.tb09326.x
• Publication Date: 2006-01-10T07:38:47Z
• Authors: Erasmo Negrete-Ab...; Rosa M García; Magda E Reyes; Delfino Godínez; Mireya Garza
• Keywords: Actinobacillus pleuropneumoniae; Polyclonal antibodies; Immunogold labelling

Actinobacillus pleuropneumoniae serotype 1 releases vesicles containing proteases and Apx toxins into the culture medium. Vesicles were concentrated by ultracentrifugation analyzed electron microscopy electrophoresis; their size ranged from 20 to 200 nm. A polyclonal antiserum raised against a purified high molecular mass secreted protease of recognized this on surface immunogold microscopy. Higher polypeptides vesicle extracts Western immunoblot, indicating that could form oligomers. However, these oligomers not active gelatin until secreted. Additionally, also present in vesicles, immunoblot an anti-serotype serum. A. antigens convalescent-phase pig sera animals infected with or 5. The release virulence factors be tissue damage mechanism swine pleuropneumonia.