TheSaccharomyces cerevisiaeYPR184wgene encodes the glycogen debranching enzyme

• DOI: 10.1111/j.1574-6968.2000.tb09410.x
• Publication Date: 2006-01-10T07:35:29Z
• Authors: Marie Ange Teste; Brice Enjalbert; Jean Luc Parrou; Jean M. François
• Keywords: Recombinant Fusion Proteins; Genes, Fungal; Molecular Sequence Data; Gene Expression; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Saccharomyces cerevisiae; Stress; Debranching enzyme; 03 medical and health sciences; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]; Animals; Humans; Amino Acid Sequence; [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology; Molecular Biology/Genomics [q-bio.GN]; 0303 health sciences; Functional analysis; Sequence Homology, Amino Acid; Glycogen Debranching Enzyme System; [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology; Protein Structure, Tertiary; Glucose; [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]; Gene Deletion; Glycogen

The YPR184w gene encodes a 1536-amino acid protein that is 34-39% identical to the mammal, Drosophila melanogaster and Caenorhabditis elegans glycogen debranching enzyme. The N-terminal part of the protein possesses the four conserved sequences of the alpha-amylase superfamily, while the C-terminal part displays 50% similarity with the C-terminal of other eukaryotic glycogen debranching enzymes. Reliable measurement of alpha-1,4-glucanotransferase and alpha-1, 6-glucosidase activity of the yeast debranching enzyme was determined in strains overexpressing YPR184w. The alpha-1, 4-glucanotransferase activity of a partially purified preparation of debranching enzyme preferentially transferred maltosyl units than maltotriosyl. Deletion of YPR184w prevents glycogen degradation, whereas overexpression had no effect on the rate of glycogen breakdown. In response to stress and growth conditions, the transcriptional control of YPR184w gene, renamed GDB1 (for Glycogen DeBranching gene), is strictly identical to that of other genes involved in glycogen metabolism.