The Spleen Is the Major Source of Antidonor Antibody-Secreting Cells in Murine Heart Allograft Recipients

• DOI: 10.1111/j.1600-6143.2012.04009.x
• Publication Date: 2012-03-15T19:02:49Z
• Authors: A. Sicard; T.W. Phares; H. Yu; R. Fan; W.M. Baldwin; R.L. Fairchild; A. Valujskikh
• Keywords: Mice, Inbred BALB C; Enzyme-Linked Immunosorbent Assay; Tissue Donors; 3. Good health; Mice, Inbred C57BL; Mice; 03 medical and health sciences; 0302 clinical medicine; Antibody Formation; Models, Animal; Animals; Heart Transplantation; Transplantation, Homologous; Spleen

Antibody-mediated allograft rejection is an increasingly recognized problem in clinical transplantation. However, the primary location of donor-specific alloantibody (DSA)-producing cells after transplantation have not been identified. The purpose this study was to test contribution allospecific antibody-secreting (ASCs) from different anatomical compartments a mouse model. Fully MHC-mismatched heart allografts were transplanted into three groups recipients: nonsensitized wild type, alloantigen-sensitized wild-type and CCR5(-/-) mice that exaggerated responses. We found previous sensitization donor alloantigens resulted development antidonor (alloAb) with accelerated kinetics. Nevertheless, numbers alloantibody-secreting serum titers IgG equivalent sensitized recipients 6 weeks Regardless recipient status, spleen contained higher donor-reactive ASCs than bone marrow at days 7-21 Furthermore, individual produced more ASCs. Taken together, our results indicate rather major source alloAb early both recipients.