Compositional Study on Rice Bran Oil after Lipase‐Catalyzed Glycerolysis and Solvent Fractionations

• DOI: 10.1111/j.1750-3841.2007.00299.x
• Publication Date: 2007-04-18T07:04:31Z
• Authors: J. H. Lee; F. Yu; P. L. Vu; M. S. Choi; C. C. Akoh; K. T. Lee
• Keywords: Hexane; Rice bran oil; Fraction (chemistry)

ABSTRACT: Rice bran oil (RBO) was modified through lipase‐catalyzed glycerolysis. After 48 h reaction, the reactant (RBO‐G, solved in hexane) containing 0.14 mg/mL of MAG, 0.19 DAG, and 0.93 TAG obtained. Extending reaction to 72 resulted 0.37 DAG with concomitant reduction (0.68 mg/mL). Two solvent fractionation methods, independent sequential fractionation, were performed acetone hexane at 0, −8, −14, or −35 °C. The fraction most unsaturated fatty acids (ΣUFA) liquid from °C (−35In) hexane, showing 88.3%ΣUFA content. Nevertheless, when yield (wt%) considered, highest amount UFA obtained 0In (liquid 0 °C) resulting 82.3%ΣUFA 97.9 wt% recovery. Normal‐phase HPLC conducted for compositional study RBO‐G. Overall, solid fractions (0SeSo) (−35InSo) contained high concentration total MAG ranging 0.94 1.35 (mg/mL).