Culturable microbiota of ranched southern bluefin tuna (Thunnus maccoyii Castelnau)

• DOI: 10.1111/jam.12286
• Publication Date: 2013-06-24T05:18:37Z
• Authors: V. Valdenegro-Vega; S. Naeem; J. Carson; J.P. Bowman; J.L. Tejedor del ...; B. Nowak
• Keywords: Thunnus

The Australian tuna industry is based on the ranching of wild southern bluefin (SBT, Thunnus maccoyii). Within this industry, only opportunistic pathogens have been reported infecting external wounds fish. This study aimed to identify different culturable bacteria present in three cohorts SBT and determine normal potential isolates from harvest fish moribund/dead Post‐mortem changes microbiota were also studied. Moribund/dead showed a greater proportion members family Vibrionaceae than harvested fish; latter presented mainly non‐Vibrio species. In spleens, Vibrio splendidus I complex was most commonly identified group among isolates, while groups isolated gills. For moribund/dead, chagasii Photobacterium damselae subsp. common gill, spleen kidney samples. Non‐Vibrio gills characterized using 16S rRNA sequencing as Flavobacteriaceae classes Gammaproteobacteria Alphaproteobacteria, genera Winogradskyella Tenacibaculum. dynamic shifts bacterial dominance gills, with spp. found similar proportions initially types related Pseudoalteromonas ruthenica prevailing after 27 h. Spleen samples little growth until 5 h post‐mortem, various Vibrio‐associated species post‐mortem. Bacterial include range potentially pathogenic that should be monitored though them yet associated disease tuna. forms foundation for future research into population dynamics under culture conditions SBT. An understanding compositions necessary evaluate effects some their health.