Hydrolysates of glycated and heat-treated peanut 7S globulin (Ara h 1) modulate human gut microbial proliferation, survival and adhesion

• DOI: 10.1111/jam.12358
• Publication Date: 2013-10-12T02:36:30Z
• Authors: M. Teodorowicz; D. Świątecka; H. Savelkoul; H. Wichers; E. Kostyra
• Keywords: intestinal microbiota; Glycosylation; Hot Temperature; Arachis; prevalence; in-vitro; chemistry; Bacterial Adhesion; 03 medical and health sciences; 0302 clinical medicine; Enterococcus faecalis; Escherichia coli; Humans; Glycoproteins; Plant Proteins; Hydrolysis; Membrane Proteins; health; Globulins; differentiation; maillard reaction-products; Antigens, Plant; proteins; Maillard Reaction; 3. Good health; Gastrointestinal Tract; Lactobacillus acidophilus; Glucose; barrier; Caco-2 Cells; allergen

Evaluation of an effect glycation Ara h 1 on proliferation and survival rate adhesion intestinal Enterococcus faecalis, Escherichia coli Lactobacillus acidophilus.Pure heated at three different temperature conditions (G37, G60 C145°C) in the presence or absence glucose was subjected to enzymatic hydrolysis. Impacts hydrolysates bacterial proliferation, Caco-2 cells mono heterogeneous cultures were studied with fluorescent techniques: DAPI, LIVE/DEAD staining FISH. Examined hindered E. Ent. faecalis simultaneous decrease their survival. Maillard reaction (MR, glycation) did not alter rate. Hydrolysates modified 60 145°C altered profile immobilized bacteria, mostly by lowering number adhering promoting bacteria from genera Enterococcus.Ara h1 processed various ways demonstrated strong modulatory adhesion.Reducing opportunistic glycated 145°C, together modulation immobilization beneficial lactobacilli enterococci, may be relevance terms physiological status barrier.