Vascular progenitor cell senescence in patients with Marfan syndrome

• DOI: 10.1111/jcmm.14301
• Publication Date: 2019-03-28T13:13:39Z
• Authors: Haiwei He; Baoqi Yu; Zipeng Liu; Gen Ye; Wei You; Yimei Hong; Qizhou Lian; Yuelin Zhang; Xin Li
• Keywords: MFN2; Senescence

Abstract Vascular progenitor cells (VPCs) present in the adventitia of vessel wall play a critical role regulation vascular repair following injury. This study aimed to assess function VPCs isolated from patients with Marfan syndrome (MFS). were control and MFS donors characterized. Compared control‐VPCs, MFS‐VPCs exhibited cellular senescence as demonstrated by increased cell size, higher SA‐β‐gal activity elevated levels p53 p21. RNA sequencing showed that several process‐related pathways including cycle significantly enriched MFP‐VPCs. Notably, expression level TGF‐β1 was much than control‐VPCs. Treatment control‐VPCs enhanced mitochondrial reactive oxidative species (ROS) induced whereas inhibition ROS reversed these effects. displayed fusion decreased fission. reduced Nonetheless, treatment mitofusin2 (Mfn2)‐siRNA inhibited TGF‐β1‐induced senescence. Furthermore, mediated AMPK signalling pathway. Our shows induces VPC mediating dynamics via