Crebanine mitigates glucocorticoid‐induced osteonecrosis of the femoral head by restoring bone remodelling homeostasis via attenuating oxidative stress

• DOI: 10.1111/jcmm.70044
• Publication Date: 2024-08-29T06:06:35Z
• Authors: Shankun Dong; Jianxun Ge; Qi Meng; Tao Yuan; Yi Wang; Yi Li; Qizhen Lu; Wenao Song; Ziqing Li; Shui Sun
• Keywords: Homeostasis; Bone remodeling

The onset of osteonecrosis the femoral head (ONFH) is intimately associated with extensive administration glucocorticoids (GCs). Long-term stimulation GCs can induce oxidative stress in both osteoclasts (OCs) and osteoblasts (OBs), resulting disturbance bone remodelling. An alkaloid named crebanine (CN) demonstrates pharmacological properties including anti-inflammation reactive oxygen species (ROS) modulation. Our objective to assess therapeutic potential CN treating ONFH elucidate underlying mechanisms. network pharmacology analysis uncovered that played a role regulating ROS metabolism. In vitro, demonstrated its ability reduce dexamethasone (DEX)-stimulated generation OCs suppress their resorptive function by downregulating level osteoclast marker genes. Concurrently, also mitigated DEX-induced damage OBs, facilitating restoration osteoblast gene expression, cellular differentiation function. These effects were achieved augmenting antioxidant system intracellular levels. Furthermore, vitro results corroborated micro-CT histological data, which showed attenuated MPS-induced mice. This study highlights counteracting GCs-induced ONFH.