Roles of GOBP1 in perception of host plant volatiles revealed by CRISPR/Cas9‐mediated mutagenesis in Spodoptera litura

• DOI: 10.1111/jen.13150
• Publication Date: 2023-06-09T16:53:31Z
• Authors: Wei‐Kang Han; Zhi‐Qiang Wei; Yi‐Lin Yang; Xiao‐Long Liu; Qi Yan; Jin Zhang; Shuang‐Lin Dong
• Keywords: Spodoptera litura; Electroantennography

Abstract The general odorant‐binding proteins (GOBPs) in Lepidoptera form a distinct OBP subclass, which exclusively has two members (GOBP1 and GOBP2) single species. GOBPs are long thought to function perception of plant odorants recently sex pheromones, but the vivo evidence is rarely provided. Here, by using CRISPR/Cas9 technique, we demonstrated that GOBP1 noctuid moth Spodoptera litura involved host volatiles not female pheromone, females less important than GOBP2 perception. First, knockout strain ( −/− ) S. was obtained technique. Second, electroantennography (EAG) tests, displayed significant reduction responses volatile farnesene, leaf discs cabbage (males only high doses), adults did show EAG major pheromone component analogues. Third, compared with females, were significantly lower (in relation WT moths) response leaves. Our results provide .