Prevalence and risk factors of potential pathogenicYersinia enterocoliticain Tunisian frozen ground beef through a shelf‐life monitoring protocol validation

• DOI: 10.1111/jfpp.16360
• Publication Date: 2022-01-08T08:44:27Z
• Authors: Sana Jaballah; Imene Fhoula; Mohamed Boumaiza; Afef Najjari; Narjes Mhajbi; Abdellatif Boudabous; Naouel Klibi; Hadda‐Imene Ouzari
• Keywords: Yersinia enterocolitica; rpoB; Yersinia; Identification

This study aims to identify and determine the prevalence of Yersinia enterocolitica in ground beef meat during conservation process. In all, 180 sample-units from three red processing companies were subjected microbial analysis. The identification rate Y. performed using conventional culture methods molecular based on analysis rpoB gene. analytical method allowed detection 25 (14%) positive units 15 strains. Biogrouping classification assignment all isolates biotype 1A. However, assessment phenotypic virulence markers revealed that 9 out strains presumptively virulent. results revealed, for first time, evidence viability within Tunisian frozen need consider investigation this bacterium standards products their safety evaluation. Furthermore, gene-based is a rapid sensitive technique products. Novelty impact statement work constitutes report conservation. highest frequency number samples was recorded at end microbiological lifetime view virulent among 1A biogroup isolates, pathogenicity bacterial group should be investigated.